Device

Part:BBa_K812012:Design

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-18)


OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 433
    Illegal NotI site found at 287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1648
    Illegal XhoI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal NgoMIV site found at 164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79


Design Notes

This part has been cloned in pSB1C3 using in-fusion and the illegal restriction site SpeI and PstI had been removed by PCR.


Source

This part has been extracted from the pNHK60 plasmid using PCR. THe pNHK60 plasmid was obtained from the riken registry.

References